Spindle View

Egg Spindle Observation

Non-invasively visualizes the oocyte's meiotic spindle with polarized-light microscopy to confirm egg maturity and inject away from the spindle during ICSI, improving fertilization and embryo quality.

JUMP01 Overview 02 Highlights 03 Process 04 Reference 05 FAQ

CHAPTER 01

Egg Spindle Observation Spindle View

The chromosomes of a mature (MII) oocyte are organized and segregated by the meiotic spindle, which is invisible under a standard light microscope.

Egg spindle observation uses polarized-light microscopy (PolScope) to non-invasively reveal the spindle's position and shape, helping confirm egg maturity and avoid the spindle during micro-injection to reduce the risk of chromosomal damage.

Recommended for

✓Advanced age or poor egg quality
✓Low fertilization rate or poor embryo quality in a previous cycle
✓Limited number of eggs retrieved; avoiding risk
✓Wishing to reduce the impact of injection on the egg

CHAPTER 02

Key points

Confirm maturity

A visible spindle indicates the egg has reached maturity (MII), aiding selection.

Precise injection

Injecting away from the spindle lowers the risk of chromosomal damage.

Non-invasive imaging

Real-time polarized-light imaging needs no staining or harm to the egg.

CHAPTER 03

Technique process

Retrieve & denude

After retrieval, remove the surrounding cumulus cells to expose the egg.

Polarized imaging

Reveal the spindle's position and shape with a polarized-light microscope.

Assess & locate

Confirm maturity, mark the spindle and plan the injection angle.

Micro-injection

Complete ICSI away from the spindle for more stable fertilization.

CHAPTER 04

Reference information

Comparison	Standard ICSI	With spindle observation
Egg maturity assessment	Based on polar-body appearance	Direct observation of the spindle
Injection positioning	Estimated from the polar body	Avoiding the actual spindle
Chromosome risk	Relatively higher	Relatively lower

CHAPTER 05

FAQ

QDoes spindle observation harm the egg?

No. It uses non-invasive polarized-light imaging with no staining or extra handling.

QIs the spindle visible in every egg?

Most mature MII oocytes show a spindle. If it is not visible, the oocyte may be immature or of lower quality.

QMust it be paired with ICSI?

This technique is mainly used to locate and assess before ICSI, so it is usually performed together with ICSI.

Time-Lapse Embryo Monitoring Incubator

Hysteroscopy

Precision meets life.

See the spindle, protect every egg's chromosomes

Our lab uses polarized-light technology for precise targeting to improve fertilization and embryo quality.

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